Function Deficiency Vitamin D sources Toxicity Contacting a doctor Summary For adults, a level of vitamin D in the blood of 20 nanograms per milliliter (ng/mL) or above is generally SPECIMEN REQUIREMENTS: Collect: 5 ml Gold (serum) or Red top tube. Separate serum (min volume required is 0.5 ml) Min. Collection: 1.0 ml whole blood Transport: Refrigerated Stability: After serum separation. Ambient: 24 hours; Refrigerated: 1 week. Frozen: 1 month Unacceptable Conditions: Room Temperature specimens older than 24 hours. According to the Endocrine Society, vitamin D deficient adults should be treated with 50,000 IU (1250 ug) of vitamin D weekly [equal to 7143 IU (179 ug) daily] for eight weeks to achieve a blood level greater than 30 ng/mL (75 nmol/L). Maintenance therapy thereafter should be at least 1500-2000 IU (38-50 ug) per day. Vitamin D deficiency (serum 25-hydroxyvitamin D [25 (OH)D] < 50 nmol/L or 20 ng/ml) is associated with unfavorable skeletal outcomes, including fractures and bone loss. A 25 Based on this evidence, the committee concluded that the bone-strengthening benefits of vitamin D plateau when blood levels (as measured by a standard vitamin D blood test) reach 12 to 16 Vitamin D tests, which include immunoassays and liquid chromatography-mass spectrometry LC/MS/MS, measure the level of 25(OH)D, the main form of circulating vitamin D. Optimal levels Vitamin D levels of 30 to 100 ng/mL are considered optimal. In people whose 25(OH)D is 20 to 30 ng/mL (50 to 75 nmol/L), treatment with 600 to 800 international units (15 to 20 micrograms) of vitamin D3 by mouth daily is usually sufficient to maintain levels in the target range. The major circulating form of vitamin D is 25-hydroxyvitamin D (25(OH)D); thus, the total serum 25(OH)D level is currently considered the best indicator of vitamin D supply Poziom 25(OH)D3 poniżej 30 µg/ml (w niektórych laboratoriach <75 nmol/l) wskazuje na niewystarczającą ilość witaminy D w organizmie. Wartości poniżej 20 µg/ml (<50 nmol/l) są stanem ciężkiego niedoboru, który powinien być leczony ściśle pod kontrolą lekarza. The Endocrine Society, the National and International Osteoporosis Foundation, and the American Geriatric Society define vitamin D deficiency as the level of 25-hydroxyvitamin (25 OH D) of less than 30 ng/mL. The Endocrine Society recommends a preferred range of 40 to 60 ng/mL. J3eKrd.